Myosin 1b regulates intestinal epithelial morphogenesis via interaction with UNC45A - Fondation pour la Recherche Médicale
Article Dans Une Revue Cell Reports Année : 2024

Myosin 1b regulates intestinal epithelial morphogenesis via interaction with UNC45A

Magda Cannata Serio
Fanny Eggeler
Marie-Thérèse Prospéri
Evelyne Coudrier
Franck Perez
Filippo del Bene

Résumé

Vesicle trafficking and the establishment of apicobasal polarity are essential processes in epithelial morphogenesis. UNC45A deficiency has been reported in a multi-organ syndrome presenting with severe diarrhea associated with enterocyte polarity defects. Myosin 1b, an actin motor able to bind membranes, regulates membrane shaping and vesicle trafficking. Here, we show that MYO1B is part of the UNC45A interactome. In the absence of UNC45A, myosin 1b is degraded and forms aggregates when proteasome activity is inhibited. In 3D Caco-2 cells, lumen formation is impaired in the absence of myosin 1b, associated with spindle orientation defects, Golgi apparatus fragmentation, and trafficking impairment. In zebrafish larvae, loss of myo1b results in intestinal bulb epithelium folding defects associated with terminal web disorganization and vesicle accumulation, reminiscent of villous atrophy. In conclusion, we show that myosin 1b plays an unexpected role in the development of the intestinal epithelium downstream of UNC45A, establishing its contribution in the gut defects reported in UNC45A patients.
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hal-04869697 , version 1 (07-01-2025)

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Céline Revenu, Corinne Lebreton, Magda Cannata Serio, Marion Rosello, Rémi Duclaux-Loras, et al.. Myosin 1b regulates intestinal epithelial morphogenesis via interaction with UNC45A. Cell Reports, 2024, 43 (12), pp.114941. ⟨10.1016/j.celrep.2024.114941⟩. ⟨hal-04869697⟩
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