Effect of high pressure homogenization on the structure and the interfacial and emulsifying properties of β-lactoglobulin - Inflammation, microbiome, immunosurveillance Accéder directement au contenu
Article Dans Une Revue International Journal of Pharmaceutics Année : 2018

Effect of high pressure homogenization on the structure and the interfacial and emulsifying properties of β-lactoglobulin

Résumé

The effect of high pressure homogenization (HPH) on the structure of β-lactoglobulin (β lg) was studied by combining spectroscopic, chromatographic, and electrophoretic methods. The consequences of the resulting structure modifications on oil/water (O/W) interfacial properties were also assessed. Moderated HPH treatment (100 MPa/4 cycles) showed no significant modification of protein structure and interfacial properties. However, a harsher HPH treatment (300 MPa/5 cycles) induced structural transformation, mainly from β sheets to random coils, wide loss in lipocalin core, and protein aggregation via intermolecular disulfide bridges. HPH-modified β lg displayed higher surface hydrophobicity leading to a faster adsorption rate at the interface and an earlier formation of an elastic interfacial film at Cβ-lg = 0.1 wt%. However, no modification of the interfacial properties was observed at Cβ-lg = 1 wt%. At this protein concentration, the prior denaturation of β lg by HPH did not modify the droplet size of nanoemulsions prepared with these β lg solutions as the aqueous phases. A slightly increased creaming rate was however observed. The effects of HPH and heat denaturations appeared qualitatively similar, but with differences in their extent.
Fichier principal
Vignette du fichier
HAL_Revised manuscript IJP 2017.pdf (1.27 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04269446 , version 1 (05-06-2024)

Identifiants

Citer

Ali Ali, Isabelle Le Potier, Nicolas Huang, Véronique Rosilio, Monique Cheron, et al.. Effect of high pressure homogenization on the structure and the interfacial and emulsifying properties of β-lactoglobulin. International Journal of Pharmaceutics, 2018, 537 (1-2), pp.111-121. ⟨10.1016/j.ijpharm.2017.12.019⟩. ⟨hal-04269446⟩
40 Consultations
3 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More